Table 1 Oligonucleotides used in this study
Oligonucleotide-name | Sequences (5′ to 3′) | |
|---|---|---|
Oligonucleotides for adaptors | ||
Adaptor-1 | Upper | TCACGACGTTGTAAAACGACGGCCAGC |
Lower | p-CATGGCTGGCCGTCGTTTTACAACGTCGTGA | |
Adaptor-2 | Upper | p-CGAGTCATAGCTGTTTCCTGTGTGAAATTGTTCCGC |
Lower | GCGGAACAATTTCACACAGGAAACAGCTATGACT | |
Adaptor-3 | Upper | TGAATTGTAATACGACTCACTATAGGGG |
Lower | p-CGCCCCTATAGTGAGTCGTATTACAATTCA | |
Primers for amplification | ||
M13 forward | GTAAAACGACGGCCAG | |
M13 reverse | CAGGAAACAGCTATGAC | |
T7 | TAATACGACTCACTATAGGG | |
Sp6 | ATTTAGGTGACACTATAGAA | |
Gene-specific primers | ||
T7 + Cyp1a1 | TGAATTGTAATACGACTCACTATAGGGGCGCAACGA | |
M13 + Cyp1a1 | CAGGAAACAGCTATGACTCGCCACTGGC | |
Rat E-cadherin forward | ATGGGAGCCCGGTGCCGCAGCTT | |
Rat E-cadherin reverse | CTCTGTGGTGATGCCGGTGGTGG | |
Oligonucleotides for custom-made glass array | ||
Mouse Cyp1a1 oligo | a-CGCCACTGGCCTTCCTGTCCTGTGACCTCT | |
Rat E-cadherin oligo | a-TGGCCCAGGGACTTCAGTGTCACTTTGGTA | |
Primers for MSRE-PCR | ||
Cyp1a1 forward | TTCCTGTCCTGTGACCTCTG | |
Cyp1a1 reverse | TTGCACCCCTGAAACATTCA | |