Fig. 3
From: Development of a novel PIG-A gene mutation assay based on a GPI-anchored fluorescent protein sensor
Flow cytometric analysis of GPI(−) cell frequency. Flow cytometry was performed using an LSRFortessa (BSL2). a Cells were collected by FSC/SSC, b, c Single cells were collected via FSC-W/FSC-H and SSC-W/SSC-H. d Dead cells were eliminated by FVS450 staining. GPI(−) (e) and GPI(+) (f) cell numbers were measured, and spontaneously mutated cells were isolated in (f) Q4