Fig. 1

The diagram summarizes the usual process and methods to generate brain organoids. hPSCs firstly divide and aggregate into EBs, which are placed in neural induction media with or without addition of pattering factors to induce neural tube like structures. After expansion, these tissues will be transferred into different devices such as spinning bioreactor, SFEBq culture plate or SpinΩ for specific brain region differentiation and further maturation