Fig. 3

Isolation of precursors for the ε-ipG formation. a Chromatogram at 280 nm (bandwidth, 100 nm). b UV spectra of peak 1 (red) and 2 (black). c UV spectra of peak 3 (red) and 4 (black). d εGua forming activity at 50 °C. e εGua forming activity at 37 °C with tBuOOH. The bars show the peak area of formed ε-ipG