Fig. 1
Formation of 8-oxodG in calf thymus DNA treated with RA and isorinic acid in the presence of metal ions and NADH. The reaction mixtures contained 100 µM/base calf thymus DNA, 20 µM CuCl2 or EDTA-Na-Fe(III), and the indicated concentrations of RA (A) or 100 µM/base calf thymus DNA, 20 µM CuCl2, 100 µM NADH, and the indicated concentrations of RA or isorinic acid (B) in 400 µl of 4 mM sodium phosphate buffer (pH 7.8) containing 5 µM DTPA. The reaction mixtures were incubated at 37 °C for 16 h. After ethanol precipitation, the DNA was digested to nucleosides with nuclease P1 and calf intestine phosphatase and then analyzed with an HPLC-ECD system. * p < 0.05 vs. 0 µM. † p < 0.05 vs. corresponding samples without NADH. ‡ p < 0.05 vs. isorinic acid or isorinic acid + NADH. Significance was analyzed using Student’s t test