Fig. 3

TMPO-AS1 interacts with let-7c-5p in NPC cells. A The candidate miRNAs were predicted with the screening conditions: CLIP Data: strict stringency (> = 5), Pan-Cancer: 8 cancer types. The impact of TMPO-AS1 depletion on expression levels of five candidate miRNAs was analyzed using qPCR analysis. B PCR analysis of let-7c-5p level in NPC cells transfected with let-7c-5p mimics or NC mimics. C Binding site between TMPO-AS1 and let-7c-5p was obtained from starBase. D and E The binding ability of TMPO-AS1 and let-7c-5p was validated using luciferase reporter assay and RNA immunoprecipitation assay. F PCR analysis of let-7c-5p level in NPC tissue and adjacent normal tissue. G PCR analysis of let-7c-5p level in NPC cells and normal nasopharyngeal cells (NP69). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001