Fig. 4

The relationship between let-7c-5p and BCAT1 in NPC. A Targets of let-7c-5p were identified with the prediction criterion of CLIP Data: strict stringency (> = 5), Pan-Cancer: 10 cancer types, Degradome: high stringency (> = 3), and Predicted Program: microT + miRanda. The mRNA levels of three genes in NPC cells overexpressing let-7c-5p were evaluated using PCR analysis. B Western blotting was conducted for quantification of BCAT1 protein level in NPC cells overexpressing let-7c-5p. C Binding area between let-7c-5p and BCAT1 was predicted with the starBase. D and E Binding capacity between BCAT1 and let-7c-5p was proved by luciferase reporter assays and immunoprecipitation assays. ^**p < 0.01, ^***p < 0.001