Fig. 1

Nucleoli numbers increase in PARP-1 stable knockdown cells. (A) The subcellular localization of nucleolin in GMU6, GMSiP, and GMRSiP human fibroblasts as revealed by epifluorescence microscopy (45X magnification). Cells were fixed and stained with rabbit anti-nucleolin monoclonal antibody and imaged. (B) Stained nucleolin puncta count was used as an indicator of the nucleoli number per cell. The percentage of cells (Y-axis) with indicated numbers of nucleoli per cell (X-axis) is shown for GMU6 (red bar), GMSiP (green bar), and GMRSiP (purple bar) cells. The data shows mean ± SEM from at least three independent experiments. (C) Samples, as in A, were processed and stained with rabbit monoclonal anti-fibrillarin antibody. (D) The images were quantified as in B. The data shows mean ± SEM from at least three independent experiments. (E) The same experiment was done using a confocal microscope (LSM 510 META Zeiss). The significance values were denoted as ‘**’ (0.001 < p ≤ 0.01)