Fig. 2

Nucleolar proteins get upregulated in PARP-1 stable knockdown cells and interact with each other. (A) Protein lysates from GMU6, GMSiP, and GMRSiP were probed with anti-nucleolin, anti-nucleophosmin, anti-fibrillarin, anti-PARP-1, and anti-actin antibodies. (B) The plot showed the respective protein expression in the three types of cells after normalization with actin. (C) Total RNA was extracted from GMU6, GMSiP, and GMRSiP cells, and nucleolin mRNA level was measured by qPCR. (D) Co-IPed samples using anti-nucleolin and anti-nucleophosmin showed the interaction of PARP-1 and nucleolar proteins with each other. The data shown was the mean ± SD of three independent experiments. The significance values were denoted as ‘*’ (0.01 < p ≤ 0.05), ‘**’ (0.001 < p ≤ 0.01), and ‘***’ (p ≤ 0.001)