Fig. 2

The design of Experiments 2 and 3. Cells were treated with saline (Neg.; negative control) or positive controls (Pos.; MMC or CP) in Experiment 2 and sample A, Neg., or Pos. in Experiment 3 for 3 h using antibiotic-free medium in the absence or presence of S9 mix, followed by recovery culturing for 21 h using media with or without antibiotics. At the end of the recovery period, the MN frequencies were determined in Experiment 2, and the pH of the medium, RICC, and MN frequencies in Experiment 3