Fig.1

A and B Analysis of rat liver toxicogenomics public data (OPEN TG-GATEs, DNA microarray) by PCA with the four genes. Discrimination of GTHCs from NGTHCs and NGTNHCs at 24 h after a single administration [Fig. 1 (A)] and 29 days after repeated administrations [Fig. 2 (B)] by PCA with the four genotoxic marker genes (Bax, Btg2, Ccng1, and Cdkn1). The gene expression profile (log2) was calculated: the mean of each control group was calculated as 0 (log2), and the ratio (exp/cont) (log2) was calculated (Appendix A.1 for 24 h and Appendix A.3 for 29 days). These numerical values were analyzed by PCA (Appendix A.2 for 24 h and Appendix A.4 for 29 days). At 24 h, Fig. 1(A) shows the results of 23 chemicals with three doses, a total of 69 points: five typical GTHCs (brown-colored, AAF, AFL, DEN, NNM, and 2NF) were discriminated from seven typical NGTHCs (yellow-colored, CLO, ETH, FEN, GEM, HEX, PHE, and WY) and 11 NGTNHCs (blue-colored, AA, ASP, CAF, CPA, CPP, DEX, DIA, IND, PBZ, THE, and TOL). At 29 days, Fig. 1(B) shows the results of 19 chemical, total of 55 points: two typical GTHCs (brown-colored, AAF-low, -middle, and -high; DEN-low and -) were discriminated from seven typical NGTHCs (yellow-colored, CLO, ETH, FEN, GEM, HEX, PHE, and WY with three doses) and 10 NGTNHCs (blue-colored, AA, ASP, CAF, CPA, CPP, DIA, PBZ, THE, and TOL with three doses and IND-low and -middle). Each group is enclosed with an optional dashed ellipse. Five GTHCs [AAF: 2- acetamidofluorene, AFL: aflatoxin B1, 2NF: 2-nitrofluorene, DEN: N-nitrosodiethylamine and NNM: N-nitrosomorpholine], seven NGTHCs [CLO: clofibrate, ETH: ethanol, FEN: fenofibrate, GEM: gemfibrozil, HEX: hexa-chlorobenzene, PHE: phenobarbital, and WY: WY-14643], and 11 NGTNHCs (mostly pharmaceutical drugs) [AA: allyl alcohol, ASP: aspirin, CAF: caffeine, CPA: chlorpheniramine, CPP: chlorpropamide, DEX: dexamethasone, DIA: diazepam, IND: indomethacin, PBZ: phenylbutazone, THE: theophylline, and TOL: tolbutamide]