Table 2 Non-animal test methods approved by OECD TG
Classification | TG | Title | Names of Test method | Published | Remarks |
|---|---|---|---|---|---|
Genotoxicity | 471 | Bacterial Reverse Mutation Test | Ames test | 1983 | Â |
472 | In vitro Mammalian Chromosomal Aberration Test | Â | 1983 | Â | |
476 | In vitro Mammalian Cell Gene Mutation Tests using the Hprt and xprt genes |  | 1984 |  | |
487 | In vitro Mammalian Cell Micronucleus Test | Â | 2010 | Â | |
490 | In vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene |  | 2015 |  | |
Skin Corrosion | 430 | In vitro Skin Corrosion: Transcutaneous Electrical Resistance Test Method (TER) | Â | 2004 | Â |
431 | In vitro Skin Corrosion: Reconstructed Human Epidermis (RHE) Test Method | EpiSkinâ„¢(VRM) | 2004 | Â | |
EpiDermâ„¢ SIT (EPI200)(VRM) | 2004 | Â | |||
SkinEthicâ„¢ RHE | 2004 | Â | |||
epiCS® | 2016 |  | |||
LabCyte EPI-MODEL24 SCT | 2019 | JaCVAM | |||
435 | In vitro Membrane Barrier Test Method for Skin Corrosion | CORROSITEX | 2006 |  | |
Skin Irritation | 439 | In vitro Skin Irritation: Reconstructed human Epidermis Test Method | EpiSkinâ„¢(VRM) | 2010 | Â |
EpiDermâ„¢ SIT (EPI200)(VRM) | 2010 | Â | |||
SkinEthicâ„¢ RHE | 2010 | Â | |||
LabCyte EPI-MODEL24 SIT | 2013 | JaCVAM | |||
epiCS® | 2019 |  | |||
Skin+® | 2019 |  | |||
KeraSkinâ„¢ SIT | 2021 | Â | |||
Serious Eye Damage and Not Requiring Classification for Eye Irritation or Serious Eye Damage | 437 | Bovine Corneal Opacity and Permeability Test Method for Identifying (i) Chemicals Inducing Serious Eye Damage and (ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage | Bovine Corneal Opacity and Permeability Test Method | 2009 | Â |
438 | Isolated Chicken Eye Test Method for Identifying (i) Chemicals Inducing Serious Eye Damage and (ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage | Isolated Chicken Eye Test Method | 2009 | Â | |
460 | Fluorescein Leakage Test Method for Identifying Ocular Corrosives and Severe Irritants | Fluorescein Leakage Test Method | 2012 | Â | |
491 | Short Time Exposure In vitro Test Method for Identifying (i) Chemicals Inducing Serious Eye Damage and (ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage | Short Time Exposure (STE) Test Method | 2015 | JaCVAM | |
492 | Reconstructed human Cornea-like Epithelium (RhCE) Test Method for Identifying Chemicals Not Requiring Classification and Labelling for Eye Irritation or Serious Eye Damage | EpiOcularâ„¢ EIT(OCL-200) | 2017 | Â | |
SkinEthicâ„¢ HCE EIT(HCE/S) | 2017 | Â | |||
LabCyte CORNEA-MODEL24 EIT | 2018 | JaCVAM | |||
MCTT HCETM EIT | 2024 | Â | |||
492B | Reconstructed Human Cornea-like Epithelium (RhCE) Test Method for Eye Hazard Identification | SkinEthicâ„¢ RHE | 2022 | Â | |
494 | Vitrigel-Eye Irritancy Test Method for Identifying Chemicals Not Requiring Classification and Labelling for Eye Irritation or Serious Eye Damage | Vitrigel®-Eye Irritancy Test | 2019 | JaCVAM | |
496 | In vitro Macromolecular Test Method for Identifying Chemicals Inducing Serious Eye Damage and Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage | Ocular Irritection (OI®) | 2019 |  | |
OptiSafe Eye Irritation Testâ„¢ | 2024 | Â | |||
467 | Defined Approaches for Serious Eye Damage and Eye Irritation | Â | 2022 | Â | |
Skin Sensitisation | 442 C | In chemico Skin Sensitisation assays addressing the Adverse Outcome Pathway key event on covalent binding to proteins | DPRA | 2015 |  |
ADRA | 2021 | JaCVAM | |||
KDPRA | 2022 | Â | |||
442D | In vitro Skin Sensitisation assays addressing the Adverse Outcome Pathway Key Event on Keratinocyte activation | KeratinoSens | 2015 |  | |
LuSens | 2022 | Â | |||
EpiSensA | 2024 | JaCVAM | |||
442E | In vitro Skin Sensitisation assays addressing the Key Event on activation of dendritic cells on the Adverse Outcome Pathway for Skin Sensitisation | h-CLAT | 2016 | JaCVAM | |
U-SENSâ„¢ | 2018 | Â | |||
IL-8 Luc assay | 2017 | JaCVAM | |||
GARD | 2023 | Â | |||
497 | Defined Approaches on Skin Sensitisation | Â | 2021 | Â | |
Immunotoxicity | 444Â A | In vitro Immunotoxicity | IL-2 Luc assay | 2023 | JaCVAM |
Phototoxicity | 432 | In vitro 3T3 NRU Phototoxicity Test | In vitro 3T3 NRU Test | 2004 |  |
495 | ROS (Reactive Oxygen Species) Assay for Photoreactivity | ROS assay | 2019 | JaCVAM | |
498 | In vitro Phototoxicity - Reconstructed human Epidermis Phototoxicity Test Method | EpiDerm™ (EPI-200) | 2021 |  | |
Skin Absorption | 428 | Skin Absorption: In vitro Test Method | Â | 2004 | Â |
Endocrine Disrupter | 455 | Performance-Based Test Guideline for Stably Transfected Transactivation In vitro Assays to Detect Estrogen Receptor Agonists and Antagonists | STTA | 2016 | JaCVAM |
VM7Luc ER TA assay | 2016 | JaCVAM | |||
456 | H295R Steroidogenesis Assay | Â | 2011 | Â | |
457 | BG1Luc Estrogen Receptor Transactivation Test Method for Identifying Estrogen Receptor Agonists and Antagonists | Â | 2012 | Deleted on 29 January 2018 | |
458 | Stably Transfected Human Androgen Receptor Transcriptional Activation Assay for Detection of Androgenic Agonist and Antagonist Activity of Chemicals | AR-EcoScreenâ„¢ | 2016 | Japan | |
AR-CALUX® | 2020 |  | |||
ARTA method using the 22Rv1/MMTV_GR-KO cell line | 2023 | Â | |||
493 | Performance-Based Test Guideline for Human Recombinant Estrogen Receptor (hrER) In vitro Assays to Detect Chemicals with ER Binding Affinity | The Freyberger-Wilson (FW) In vitro Estrogen Receptor (ER) Binding Assay Using a Full Length Human Recombinant Erα | 2018 |  | |
The Chemical Evaluation and Research Institute (CERI) In vitro Estrogen Receptor Binding Assay Using a Human Recombinant Ligand Binding Domain Protein | 2018 | Japan |