Fig. 2

Repair kinetics of DSBs in X-ray-irradiated cells. A Typical neutral comet images. The indicated cells were irradiated with and without X-rays, incubated in medium for 180 min, and analyzed for DSBs using neutral comet assays. The images below the X-rays show the samples prepared immediately after X-ray irradiation (0 min repair). B Tail moments (raw data) of irradiated cells in neutral comet assays. In total, we assessed 50 cells/sample and conducted experiments in triplicate for each cell type. Tail moments of different cells of each cell type from three experiments are plotted vertically in three separate columns. Lines indicate median tail moments. C Median tail moments were quantified for 50 cells/sample/experiment and standardized to those after 0 min of repair, bars on X-rays. DSBs remaining are presented as a percentage of the remaining damage. Error bars are standard deviations of the mean of three independent assays. Significant differences were identified using an ANOVA: n.s. = not significant. Median tail moments before standardization are shown in Fig. S1A