Fig. 1
MMP-1 induction after exposure to UVA or CSS. (A) (C) MMP-1 and COL1A1 mRNA expression 24 h after exposure to UVA or CSS. ASF-4-1 cells were irradiated with UVA (15 or 30 kJ/m2) or treated with CSS (2 or 3%). After incubation for 24 h, RT-qPCR was performed as described in Materials and Methods. (B) Protein levels of MMP-1 24 h after exposure to UVA or CSS. ASF-4-1 cells were irradiated with UVA (up to 60 kJ/m2) or treated with CSS (up to 5%). After incubation for 24 h, cells were lysed and Western blotting was performed as described in Materials and Methods. Actin was used as a standard for the equal loading of proteins for SDS–PAGE. *p < 0.05. ***p < 0.001 (vs. untreated (UVA 0 kJ/m2 or CSS 0%))