Fig. 4
Histone acetylation by UVA or SB, and MMP-1 induction after treatment with CSS. (A) Histone acetylation after UVA irradiation. ASF-4-1 cells were irradiated with UVA (up to 15 kJ/m2) and cultured for 8–24 h. Western blotting was performed and histone acetylation was analyzed using three types of histone acetylation antibodies. Actin was used as a standard for the equal loading of proteins for SDS–PAGE. (B) MMP-1 and COL1A1 mRNA expression 24 h after combined exposure to SB and CSS. ASF-4-1 cells were treated with SB (3 mM), followed by treatment with CSS (2%). After incubation for 24 h, RT-qPCR was performed. *p < 0.05, **p < 0.01, ***p < 0.001 (vs. SB 0 mM). (C) Protein levels of global histone acetylation and MMP-1 24 h after combined exposure to SB and CSS. ASF-4-1 cells were treated with SB (up to 10 mM) for 24 h, followed by treatment with CSS (up to 4%). After incubation for 24 h, cells were lysed and western blotting was performed